Preparation of internal quality control substance of real-time RT-PCR to quantitative influenza virus RNA and drawing quality control chart
10.3760/cma.j.issn.1003-9279.2014.01.019
- VernacularTitle:用于荧光定量检测流感病毒RNA质控品的制备及质控图的绘制
- Author:
Liqi LIU
1
;
Xiang ZHAO
;
Leying WEN
;
Junfeng GUO
;
Hejiang WEI
;
Dayan WANG
;
Yuelong SHU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Influenza A virus;
RNA;
Quality control
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(1):55-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To draw a Levey-Jennings quality control chart using influenza virus RNA as internal quality control substance.Method Recent popular representative strains of influenza virus were selected and extracted RNA.Make series 10 folds dilution and take different dilution to do quantitative RTPCR.Three parallel wells of each dilution were done.High Ct value and low Ct value corresponding dilution of RNA were selected and detected continuously twenty times.Mean Ct value (x) and standard deviation (s) were calculated.Levey-Jennings quality control chart were drew by using Excel software.Result Levey-Jennings quality control charts of three influenza virus subtype were drew and each internal quality control substance was in control.Conclusion RNA quality substance of each subtype has good stability and preparation simple which is convenient to draw quality control chart and suitable as internal quality control substance for PCR laboratory.
