Regulatory effect of Zhuanggu Zhitong Capsule on Treg/Th17 balance in rats with osteoporosis induced by ovariectomy
10.3969/j.issn.1006-2157.2016.07.007
- VernacularTitle:壮骨止痛胶囊对去卵巢骨质疏松大鼠骨组织Treg／Th17平衡的调节作用
- Author:
Ying ZENG
1
;
Weijuan LI
;
Wenjuan ZHANG
;
Jinping LI
;
Xiaoming LEI
;
Xinmin MO
Author Information
1. 湖南中医药大学第一附属医院 湖南410007
- Keywords:
Zhuanggu Zhitong(Bone-strengthening Pain-relieving)Capsule;
Treg/Th17;
postmenopa-usal osteoporosis;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(7):555-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-postmenopausal osteoporosis mechanism of Zhuanggu Zhi-tong Capsule ( Bone-strengthening Pain-relieving Capsule , ZGZTC) in regulating Treg/Th17 balance in bone tissue .Methods Seventy-two 8-month-old SD female rats were allocated randomly into sham oper-ation group, model group, estradiol group (0.21 mg/kg), ZGZTC high-dose group (1.28 g/kg), ZGZ-TC medium-dose group (0.64 g/kg) and ZGZTC low-dose group (0.32 g/kg).There were 12 rats in each group .Bilateral ovariectomy was performed on rats in all groups except for the sham operation group.After administration for 18 weeks, all rats were anaesthetized intraperitoneally with hydrazine hy-drate .The content of E 2 , IL-10 and IL-17 A was measured with ELISA in left femur .Gene expression of Foxp3, RORγt , IL-17A and IL-10 in right femur was detected by using RT-PCR, and the number of cell with RORγt or Foxp3 was counted with immunohistochemistry in left tibia .Results Zhuanggu Zhitong Capsule significantly increased E 2 and IL-10 levels in bone tissue (P<0.01), reduced the level of IL-17A in bone tissue (P<0.05 or P<0.01).It significantly increased the Foxp3 and IL-10 mRNA ex-pression and inhibited the RORγt mRNA expression in bone tissue (P<0.05).The percentage of cell with Foxp3 was significantly increased by ZGZTC in bone tissue ( P<0 .05 ) , and the percentage of cell with RORγt was significantly reduced (P<0.05 or P<0.01).The ratio of Foxp3 to RORγt presented a positive correlation with the E2 level (r=0.990 5,P<0.01).Conclusion Zhuanggu Zhitong Capsule could downregulate the number and function of Th 17, upregulate the number and function of Treg in bone tissue in ovariectomized rats partly via improving the E 2 level.
