Amplification of nearly-complete sequence HBoV1 and recombination analysis among HBoV1-4
10.3760/cma.j.issn.1003-9279.2014.01.010
- VernacularTitle:人博卡病毒1型的近似全基因组扩增及HBoV1-4重组分析
- Author:
Huandi CUI
1
;
Yu JIN
;
Guangcheng XIE
;
Weixia CHENG
;
Qing ZHANG
;
Zhaojun DUAN
Author Information
1. 兰州大学第一医院儿科
- Keywords:
Bocavirus,human;
Phylogeny;
Recombination,genetic
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(1):28-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To amplify the nearly-complete sequence of human Bocavirus 1 (HBoV1)and analyze the recombination relationship among HBoV1-4.Methods Five fragments of HBoV1 genome were amplified by PCR from the HBoV1 single positive nasopharyngeal aspirates,then the five fragments were sequenced and analyzed through the blast in nucleotide acid database.The sequence assembly was conducted by DNAMAN,the homogeneous analysis was performed among the HBoV1 sequences that have been published in nucleotide acid database and then created the phylogenetic tree.At last,the recombination relationship was analyzed among HBoV1-4.Results A total 5287 bp length sequence was amplified in this study,which was the nearly-complete sequence of HBoV1 that was named HBoV1-NC.HBoV1-NC had the closest relationship with Chongqing strain,however,had the farthest relationship with Guangzhou strain through the homogeneous analysis.HBoV3 may be a recombinant derived from HBoV1 and HBoV4,HBoV4 may be a recombinant derived from HBoV2 and HBoV3 through recombination analysis of HBoV1-4.Conclusion The nearly-complete sequence HBoV1-NC was amplified in this study and HBoV1-4 had the recombination relationship.
