Optimization of ultrasonic-assisted extraction of total flavonoids from Lomatogonium rotatum by response surface analysis
10.3969/j.issn.1006-2157.2016.06.013
- VernacularTitle:响应面法优化蒙药肋柱花总黄酮超声提取工艺
- Author:
Ting LIU
1
;
Youheng GAO
;
Chunjuan GE
;
Changhua YAN
;
Yaoqiu LIANG
;
Xiao LI
Author Information
1. 内蒙古医科大学药学院 内蒙古010110
- Keywords:
Lomatogonium rotatum;
total flavonoids;
ultrasonic-assisted extraction;
response surface methodology
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(6):502-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize ultrasonic-assisted extraction process of total flavonoids from Lomatogonium rotatum by using single factor experiment and response surface methodology,so as to provide the theoretical evidence for the exploitable and utilization of flavonoids in Lomatogonium rotatum. Methods Ethanol solution was selected as the extraction solvent.Basedonthesinglefactorexperiment, three independent variables of ethanol volume fraction (X1 ),material to liquid ratio (X2 )and sonication time(X3)with the response value of the content of total flavonoids(Y)were designed for Box-Behnken central composite experiment,then a quadric regression equation was established to predict the yield of totalflavonoids.Results The optimum extraction process was 65% ethanolsolution(V/V)withliquidto material ratio of 20 mL/g for 20 minutes.The actual yield of total flavonoids were 2 1 .26 mg/g,very close to the predictive values.The binomial fitting multiple correlation coefficient (r)is 0.956 8.Conclusion Optimizing the extraction process of total flavonoids of Lomatogonium rotatum by response surface methodology was proved to be stable,reasonable and feasible.
