The cervical mucosal exfoliated cells telomerase quantitative research in HPV infection, cervicitis,CIN and cervical cancer patients
10.3760/cma.j.issn.1003-9279.2014.01.008
- VernacularTitle:HPV感染、宫颈炎、CIN及宫颈癌患者的宫颈黏膜脱落细胞端粒酶定量研究
- Author:
Zhuneng FU
1
;
Ronghui WU
;
Haifang SUN
;
Xianfang WANG
;
Lanqing SUN
;
Linhai CHEN
Author Information
1. 322000,浙江义乌市中心医院
- Keywords:
Telomerase;
Cervix diseases;
Papillomavirus human;
Cervical cancer
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(1):23-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective Studies show that women infected with human papillomavirus (HPV)induced cervicitis,cervical intraepithelial neoplasia (CIN) and on to develop cervical cancer their cervical mucosal exfoliated cells telomerase activity changes,to explore its clinical value in screening and early diagnosis and treatment of female patients with cervical cancer.Methods Elections of HPV testing negative control group 26 cases,HPV-positive group 28 cases,HPV-positive cervicitis group of 27 cases,HPV-positive CIN group 26 cases,24 cases of cervical cancer,HPV-negative cervicitis group 26 cases,HPV-negative CIN group of 23 cases of seven test group,cervical mucosa cytology specimens collected in 293 cells as positive standards for real-time fluorescence quantitative detection of telomerase TRAP Quantitative value of telomerase activity.Results Normal control group,ranging from 0 to 3.15,average 0.36; HPV-positive group,ranging from 0 to 21.6,with an average 1.98,＞ 10,3 cases; HPV-positive cervicitis group,ranging from 0 to 71.5,with an average 4.88,＞ 10,4 cases; HPV-positive CIN group,ranging from0 to 407,with an average 20.8,＞ 10,5 cases; cancer group,the range 1.2-5.5 × 106,median 346,where ＜ 10.4 cases; HPV-negative cervicitis group,ranging from 0 to 66.6,with an average 4.19,＞ 10,3 cases; HPV-negative CIN group,ranging from 0 to 158,with an average 14.2.Statistical analysis of cervical cancer group and the other groups are statistically significant (P ＜0.01),HPV-positive CIN group compared with the control group was statistically significant (P ＜ 0.05) the rest of the comparison between two groups was not statistically significance (P ＞ 0.05).Conclusions Women infected with HPV and cause cervicitis,CIN stage,the cervical mucosa telomerase activity gradually increased and in cervical cancer stage increased the most significant.And therefore take cells exfoliated cervical mucosa quantitative detection of telomerase activity can be used as effective method in screening and early diagnosis for cervical cancer in women.
