Simultaneous determination of the content of three active components in different parts of Isodon excisoides by HPLC
10.3969/j.issn.1006-2157.2016.06.011
- VernacularTitle:HPLC法同时测定拟缺香茶菜不同部位中3个活性成分的含量
- Author:
Liping DAI
1
,
2
;
Meng ZHAO
;
Jiaojiao ZHAO
;
Mingsan MIAO
;
Mengqi LIU
;
Lingxia ZHANG
;
Suiqing CHEN
Author Information
1. 河南中医药大学 河南450046
2. 呼吸疾病诊疗与新药研发河南省协同创新中心
- Keywords:
Isodon excisoides;
different parts;
index components;
HPLC
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(6):494-497,501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of simultaneous determination of blumenolA,kamebanin and henryin in different parts of Isodon excisoides by using HPLC.Methods The parameters of HPLC were listed as the following:YMC C18(4.6 ×250 mm,5 μm)reversed-phase LC column;acetonitrile-water (32∶68)isocratic elution;flow rate at 1 .0 mL/min;temperature of 30 ℃;UV detection wave-lengthof230nm.Results BlumenolA,kamebanin and henryin showed a good linearity in the range of 0.001 11 ~0.022 2 g/L (r=0.999 7),0.001 855 ~0.037 1 g/L (r=0.999 5),0.013 25 ~0.265 0 g/L (r=0.999 7)respectively.The average recovery of the three ingredients were 99.58% (blumenol A),99.51% (kamebanin)and 102.78% (henryin)with RSD 2.29%,1.49%,1.57% correspond-ingly.The content of three index components in different parts of Isodon excisoides varied,with the largest contentinleaves.Conclusion Themethodestablishedissensitive,accurateandeffective,whichcan be used for determination of the content of three active components in different parts of Isodon excisoides.
