Quality representation and correlation analysis of Ligustrum lucidum Ait. based on characteristic spectrum of drug system
10.3969/j.issn.1006-2157.2016.06.009
- VernacularTitle:基于酚类成分特征图谱的女贞子质量表征研究
- Author:
Limin ZHAO
1
;
Guangying LU
;
Shujuan YANG
;
Shuya XU
;
Ping PENG
;
Gang LI
;
Huanjuan LI
;
Duo FENG
;
Changming YANG
;
Renbing SHI
Author Information
1. 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102
- Keywords:
Nüzhenzi;
characteristic spectrum;
quality representation;
salidroside;
tyrosol;
specnu-ezhenide;
quercetin
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(6):480-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish qualityrep resentation and correlation analysis method of Ligustrum lucidum Ait.(Glossy Privet Fruit,Nüzhenzi)based on characteristic spectrum of drug system,so as to evaluate thequality of Nüzhenzi decoction pieces accurately and comprehensively.Methods Usinges-tablished HPLC-PDA method to represent the quality of Nüzhenzi characteristic spectrum of drug system. Then the qualitative representation of 14 batches of Nüzhenzi decoction pieces was based on the quantity and type of characteristic peaks of specific chromatograms,the quantitative representation of 14 batches of Nüzhenzi pieces was based on the content of four characteristic index components including salidroside, tyrosol,specnuezhenide and quercetin and the sum of iridoids (using specnuezhenide for representation)and flavonoids (using quercetin for representation ), then correlation analysis was based on the representation results of quantity and quality respectively in 14 batches of Nüzhenzi pieces referenced to thebenchmarkdecoctionpiece.Results TakingLot.S10as the benchmark,in Nüzhenzi HPLCspecific chromatogram there were 1 1 characteristic peaks which were common in all batches,including two benzene glycosides peaks ,five iridoids peaks,four flavonoids peaks.The contents of effective index components in batch S14,S12,S5,S8,S9,S6 and S11 were higher,with the highest correlation in S14, S12,S5,S8,S9 and S10,so the quality of S5,S8,S9,S12,and S14 were superior to other batches. Conclusion The evaluation model of qualityrep resentation and correlation analysis of Nüzhenzispecific chromatogram concerning on correlation of the overall quality and the effectiveness of the application,can effectively and accurately evaluate the quality of Nüzhenzi.
