Effect of hepatitis C virus core protein and NS4B on the proliferation of HepG2 cells
10.3760/cma.j.issn.1003-9279.2014.01.001
- VernacularTitle:HCV核心蛋白与NS4B对HepG2细胞增殖的影响
- Author:
Xiaohua JIANG
1
;
Yutao XIE
;
Dongcui ZHANG
;
Chuang LEI
;
Lingling LIU
Author Information
1. 湖南长沙 410087 中南大学湘雅医院感染科
- Keywords:
Hepatitis viruses;
Viral core proteins;
Viral nonstructural proteins;
Cell division
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(1):1-3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hepatitis C virus (HCV) core protein and nonstructural protein 4B(NS4B) on the proliferation of HepG2 cells and its possible mechanism.Methods The two recombinant plasmid pcDNA3.1 (-)Core and pcDNA3.1 (-) NS4B were transiently transfected respectively and co-transfected into HepG2 cells by lipofectamine, simultaneously HepG2 cells transfected with pcDNA3.1 (-) and untransfected HepG2 cells were used as control.The expression of mRNA and protein of HCV Core,NS4B,Wnt1,β-catenin,c-myc and CyclinDl in the cells of each group were detected by RT-PCR and Western blot respectively.Cell proliferation changes were measured by MTT assay and plate colony formation assay.The cell cycle distribution was tested by flow cytometry.Results ①HCV Core or/and NS4B mRNA and protein were expressed successfully in the HepG2 cells transfected with pcDNA3.1 (-)Core,pcDNA3.1 (-)NS4B alone or in combination.② The relative expression levels of mRNA and protein of Wnt1,β-catenin,c-myc and CyclinD1 were higher in the cells transfected with pcDNA3.1 (-) Core,pcDNA3.1 (-) NS4B alone or in combination than those in the cells transfected with pcDNA3.1 (-) and untransfected HepG2 cells(P ＜0.01).③Compared with the HepG2 cells transfected with pcDNA3.1 (-)and untransfected,cell viability,cloning efficiency and the percentage of cells at S and G2/M phases were significantly increased in the cells transfected with pcDNA3.1 (-) Core,pcDNA3.1 (-) NS4B alone or in combination (P ＜ 0.01).Conclusion HCV core protein and NS4B can accelerate cell cycle progression and promote cell proliferation of HepG2 cells probably through enhancement of Wntl,β-catenin,c-myc and CyclinD1 expression.
