Evaluation of two commercial cytomegalovirus IgG ELISA diagnostic kits
10.3760/cma.j.issn.1003-9279.2013.06.027
- VernacularTitle:两种巨细胞病毒IgG抗体ELISA诊断试剂性能评价
- Author:
Jun-Mei YANG
1
;
Jin-Xiang LI
;
Zhen-Xin GUO
;
Ding ZHAO
Author Information
1. 450053,郑州市儿童医院
- Keywords:
Cytomegalovirus;
Immunoglobulin G;
Reagnent kits,diagnostic;
Evaluation studies
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(6):477-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the two commercial cytomegalovirus IgG ELISA diagnostic kits.Methods Anti-CMV quality control panel QTC711 and seroconversion Panel PTC901 from BBI,seroconversion panel SCP-CMV-001 (RP-003) and SCP-CMV-002 (RP-019) from BIOMEX,and 2163 samples from three population groups were detect by the two kits.The inconsistent sample were retest by Diasorin CMV-IgG ELISA kit and Mikrogen recomblot CMV-IgG kit.Results Three seroconversion panel result show that the average detected positive time of A kit is 25 days earlier than B kit,the sensitivity of A kit is same as Abbott Imx CMV.The two kits detect 607 pregnant woman samples,8 were inconsistent,the coincidence rate is 98.68％; 512 outpatients samples,7 were inconsistent,the coincidence rate is 98.63％ ; 1044 Pediatric population samples,74 were inconsistent,the coincidence rate is 92.91％ ; the coincidence rate of Pediatric group is lower than other two groups.161 negative samples detect by A and B kits,and 89 samples positive by A kit but negative by B kit,were retest by Diasorin CMV-IgG ELISA kit,the positive coincidence rate of A and Diasorin kit is 100％,negative coincidence rate is 93.64％,total coincidence is 95.62％ ; the negative coincidence rate of B and Diasorin kit is 100％,total coincidence is 68.92％,78 samples were negative by B kit.12 A and Diasorin kit detect inconsistent samples and 6 consistent samples were retest by recomblot CMV-IgG,14 results were positive,4 results were negative.Conclusion The detection window period of A kit is shoter than B kit.The coincidence rate of A and B kit for pregnant women and outpatient population is higher than pediatrie population.The inconsistent samples were retest by imported kits,A kit show high consistent with imported kit than B kit.
