Study on clinical value of HBV large envelope protein detected by up-converting phoshor immune chromatography assay
10.3760/cma.j.issn.1003-9279.2013.06.024
- VernacularTitle:上转发光免疫层析法定量检测乙型肝炎病毒外膜大蛋白的临床价值研究
- Author:
Jin GAO
1
;
Ou WU
;
Ai-Fang XU
;
Miao-Chan WANG
;
Li-Zhi XUE
;
Wen-Yan YU
;
Hai-Ying NIU
Author Information
1. 310023,杭州市西溪医院
- Keywords:
Viral envelope proteins;
Immunochemistry;
Virus replication;
Up-converting phosphor technology
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(6):469-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application value of the up-converting Phoshor technology immunochromatography for HBV large envelope protein (HBV-LP) quantitative determination strip in hepatitis B patients.Methods Serum HBV-LP was detected by a new UPT-based immunochromatograhpic technology,and HBV DNA was quantitively detected by real time fluorescent quantitation polymerase chain reaction (RT-PCR),HBV five serum markers were detected by chemiluminescence method.Results In 500 cases of patients with hepatitis B,HBV-LP and HBV DNA positive rates were 58.0％ and 42.2％ respectively,there was significant difference between the positive rate of HBV DNA and that of HBeAg(P ＜ 0.01); In 215 cases of HBeAg negative specimens,the positive rates of HBV DNA and HBV-LP were 29.3％ and 37.2％ respectively,the difference was statistically significant (P ＞ 0.05); and HBeAg positive rate was 57.0％,there was significant difference between the positive rate of HBV DNA and that of HBeAg (P ＜ 0.01).Conclusion HBV-LP detected by UPT method can be used for the evaluation of viral replication and prognosis of patients with HBeAg negative and HBV DNA low copies patients.Combing detection of HBV DNA,HBV-LP and HBeAg is conducive to the judgment of HBV replication level and determination of antiviral treatment end point.
