Improving the quality standard for Jinlian Qingre Capsule

VernacularTitle:金莲清热胶囊质量标准研究?
Author: Rui DIND ¹ ; Kai HE ; Haixu ZHANG ; Qing WANG
Author Information

1. 宁夏药品检验所宁夏750001
Keywords: TLC; HPLC; Jinlian Qingre Capsule; vitexin; quality standard
From: Journal of Beijing University of Traditional Chinese Medicine 2016;39(5):390-394
CountryChina
Language:Chinese
Abstract: Objective To improve the quality standard of Jinlian Qingre Capsule. Methods Chinese Globeflower Flower(Trollii Chinensis Flos, Jinlianhua),Dyers Woad Leaf(Folium Isatidis, Daqingye), Figwort Root ( Srophulariae Radix, Xuanshen ) and Bitter Apricot Seed ( Semen Armeniacae Amarum, Kuxingren) in Jinlian Qingre Capsule were identified using thin layer chromdograph ( TLC) . The content of vitexin was detected using HPLC with a Phenomenex Luna C18 (2)100A column(250 mm × 4. 6 mm,5μm), a mobile phase of acetonitrile- 0. 4% ammonium acetate(15∶85)at the flow rate of 1. 0 mL/min, detection wave length at 340 nm and the column temperature at 25 ℃. Results Main spots of Chinese Globeflower Flower,Dyers Woad Leaf,Figwort Root and Bitter Apricot Seed in TLC were clear and sepa-rated well. A good linearity was obtained for vitexin in the range of 0. 06~0. 57 μg(r=0. 999 9)with mean sample recovery rate of 99 . 7% and RSD of 2 . 1%. Conclusion Method of TLC and HPLC estab-lished in this study which appears to be highly specific,accurate and repeatable can be used in the quality control of the Jinlian Qingre Capsule.