Mutations in the NS5A region of hepatitis C virus genotype 1b and correlation with response to pegylated interferon alpha-2a and ribavirin combination therapy
10.3760/cma.j.issn.1003-9279.2013.06.020
- VernacularTitle:基因1b型丙型肝炎病毒NS5A区变异及其与聚乙二醇干扰素α-2a联合利巴韦林治疗应答的相关性
- Author:
Juan-Juan FU
1
;
Wei-Jing KONG
;
Dong JIANG
;
Lai WEI
;
Xiu-Cheng PAN
Author Information
1. 徐州医学院附属医院感染性疾病科
- Keywords:
Hepatitis C;
Interferon Alfa;
Polyethylene glycols;
Ribavirin
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(6):458-460
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the variability in the NS5A region among patients with hepatitis C virus(HCV) genotype 1 b affect the response to pegylated interferon alpha-2a(PEG-IFNα-2a) and ribavirin(RBV) combination therapy.Methods We retrospectively analyzed 15 patients (7 with rapid virological response and 8 non-response to therapy) with chronic HCV genotype-1b infection treated with PEG-IFNα-2a plus RBV.The entire NS5A region was amplified by reverse transcription (RT)-PCR followed by cloned sequenced.Results No single amino acid and motif substitution were associated with different responses to therapy in any of the NSSA regions.No significant differences were found in the number of mutations in the full-length NS5A,ISDR,PKRBD,and IRRDR.The number of mutations in the V3 region (aa 2356-2379)was higher in patients with rapid virological response than in patients with non-response (5.3 ± 0.5 and 3.4 ± 0.6 respectively,P =0.03).Conclusion The number of amino acid mutations in the V3 region was correlated with RVR to PEG-IFNα-2a plus RBV therapy in HCV-1b patients.
