Simultaneous determination of residual amount of organophosphorus and pyrethroid pesticides in 13 Miao ethnomedicines using UAE-SPME-GC
10.3969/j.issn.1006-2157.2016.03.015
- VernacularTitle:UAE-SPME-GC 法对13种苗药中有机磷及拟除虫菊酯类农药残留量的同时测定
- Author:
Maoqiu HE
1
;
Jinliang LIANG
;
Changyan XU
;
Wei ZHOU
;
Xiaoyan HAO
;
Yan LIANG
Author Information
1. 贵州医科大学药学院 贵州 550025
- Keywords:
solid-phase microextraction;
gas chromatography;
ultrasound-assisted extraction (UAE);
Miao ethnomedicines produced in Guizhou;
organophosphorus pesticide;
pyrethroid pesticide
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(3):252-258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new gas chromatography (GC)method coupled with ultrasound-assisted extraction (UAE)and solid-phase microextraction (SPME)for rapid determination of seven kinds of organophosphorus pesticide residues and three kinds of pyrethroid pesticide residues in 13 Miao ethnomedicines produced in Guizhou province.Methods Japanese Honeysuckle Flower (Flos Lonicerae Japonicae,Jinyinhua)was the optimization object.SPME was used directly for enrichment of extracts obtained by ultrasonic-assisted solvent.Then gas chromatography-electron capture detector was used for the determination.Results Under optimum conditions,seven kinds of organophosphorus pesticides showed a good linear relationship at the range from 5 to 500 μg/L,and three kinds of pyrethroid pesti-cides did at the range from 2.5 to 250 μg/L.The recovery was from 85.6 % to 112.7 % with the RSD 3.2% ~9.8%.The limit of detection was from 0.02 to 0.33 μg/L.Conclusion UAE-SPME-GC established is an easy,environmental-friendly and rapid method for simultaneous determination of residual amount of 10 organophosphorus and pyrethroid pesticides in Miao ethomedicines.This method with directly enriching and sampling,was a simplified sample pretreatment procedure without purification process.
