Comparison of immunological effect induced with DC-LMP2 and rAd-LMP2
10.3760/cma.j.issn.1003-9279.2013.06.013
- VernacularTitle:DC-LMP2与rAd-LMP2所诱导的细胞免疫应答效果研究
- Author:
Hai-Jun DU
1
;
Zhan WANG
;
Xiu-Xia WEI
;
Ling ZHOU
;
Shu-Qing YE
;
Yi ZENG
Author Information
1. 100052北京,中国疾病预防控制中心病毒病预防控制所,传染病预防与控制国家重点实验室
- Keywords:
Dendritic cells;
Herpesvurus 4,human;
Immunity,intramuscle;
Viral envelope proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(6):437-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare immunological effect induced with DC-LMP2 and rAd-LMP2 in BALB/c mice.Mehtods Bone marrow cells were separated from BALB/c mice,and induced into murine dendritic cells (mDCs) cultured by RPMI 1640 medium supplemented with 10％ fetal calf serum and recombinant murine cytokines in vitro.mDCs were infected by recombinant adenovirus including latent membrane protein Ⅱ (LMP2) gene (rAd-LMP'2) of Epstein-Barr virus(EBV) at a dose of 100 multiplicity of infection (MOI),and matured mDC-LMP2 was harvested at 48 hours later.The LMP2 expression in mDCs was detected by immunoenzyme.BALB/c mice were immunized with PBS DC-LMP2 and rAd-LMP2 by intramuscular injection at 0,2,4 weeks.LMP2 specific cytotoxic lymphocytes (CTLs) in BALB/c mice were detected at 5th week and 8th week with enzyme-linked immunosorbent spot (ELISPOT).Results mDCs from bone marrow cells were harvested.LMP2 was detected in DC-LMP2.LMP2 specific CTLs were stimulated by both DC-LMP2 and rAd-LMP2 and decreased with time elapsed.LMP2 specific CTLs stimulated by rAd-LMP2 were higher than that by DC-LMP2 in BALB/c mice.Conclusion Immunization with rAd-LMP2 by intramuscular injection was more fitful than that with DC-LMP2 in BALB/c mice.
