Toll-like receptor-8 gene rs3764880 polymorphism and ischemic stroke risk in patients of Han Chinese males
10.3969/j.issn.1006-2157.2016.02.007
- VernacularTitle:Toll样受体8基因rs3764880多态性与汉族男性缺血性中风的关联性研究
- Author:
Lian GU
1
;
Jinying ZHOU
;
Jinjing TAN
;
Baoyun LIANG
;
Yan YAN
;
Qing CHEN
;
Juanjuan XIE
;
Nong TANG
Author Information
1. 广西中医药大学第一附属医院脑病科 广西530023
- Keywords:
ischemic stroke;
traditional Chinese medicine pattern;
Toll-like receptor-8;
single nucleotide pol-ymorphism;
inflammatory cytokines
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;(2):122-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association between Toll-like receptor-8(TLR8) genetic polymorphisms and susceptibility to ischemic stroke (IS) in patients of different traditional Chinese medicine (TCM) pat-terns. Methods 311 IS patients of wind-phlegm stasis-obstruction pattern, 284 IS patients of qi-deficiency blood-stasis pattern and 605 controls were recruited. The real-time PCR technology was used to detect TLR8 gene expression levels. TLR8 rs3764880 single nucleotide polymorphism(SNPs) were genotyped using the Se-quenom Mass ARRAY platform. Levels of inflammatory cytokines were measured by using ELISA. Statistical analyses were carried out by using PLINK and SPSS 16. 0 software. Results TLR8 gene variant rs3764880 polymorphism was significantly associated with wind-phlegm stasis-obstruction pattern in IS males patients in the allele model (P<0. 05). In IS male patients of qi-deficiency blood-stasis pattern , level of IL-8 was high-er in the GG genotype carriers than the AA+AG genotype carriers (P<0. 05). The GG+AG genotype carri-ers had higher levels of TNF-α and IL-8 than the AA genotype carriers ( P <0. 05 ) . TLR8 gene variant rs3764880 polymorphism was significantly associated with the levels of TC (βb = -0. 03, Padj =0. 001) and LDL (βb= -0. 25, Padj =0. 018) in additive model in qi-deficiency blood-stasis pattern in Han Chinese IS male patients. Conclusion TLR8 gene rs3764880 polymorphism might be associated with the susceptibility to IS in patients of wind-phlegm stasis-obstruction and affect inflammatory reaction and lipid metabolism in Han Chinese males of qi-deficiency blood-stasis pattern.
