Effect of different temperature on the susceptibility of Aedes albopictus for transmission of dengue 2 virus
10.3760/cma.j.issn.1003-9279.2012.03.008
- VernacularTitle:温度对白纹伊蚊传播登革2型病毒易感性影响的研究
- Author:
Fang-Zhen XIAO
1
;
Yi ZHANG
;
Si HE
;
Wen-Qi SHI
;
Han-Guo XIE
;
Yan-Qin DENG
;
Xiao-Nong ZHOU
;
Yan-Sheng YAN
Author Information
1. 福建省疾病预防控制中心
- Keywords:
Aedes;
Dengue virus;
Temperature
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(3):186-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different temperature on the susceptibility of Aedes albopictus for transmission of dengue 2 virus.Methods Mosquitoes were orally infected with dengue virus type 2 suspension,then fully blood-engorged mosquitoes were separated and transferred to new cartons and sustained at 18,21,26,31,33℃ and 36℃ with 10％ sucrose.Immunofluorescence assays were used to detect antigen of dengue virus type 2 in the head,salivary gland and thorax-abdomen,and calculated the disseminated infection rate of mosquitoes.Results For mosquitoes held at 18℃,the infection rate of thoraxabdomen was 8％,infection in head and salivary gland were not detected until 25d.Maximum infection rate of thorax-abdomen was attained at 31℃,the highest infection rate of head and salivary gland existed at 33℃,however,the infection rate of mosquitoes at 36℃ was significantly decreased compared with that at 33℃.The lowest disseminated infection rate was zero at 18℃,while the highest disseminated infection rate was 100％ at 36 ℃.Conclusion As the temperature went up,the infection rate showed a tendency of ascending first and descending later.Dengue virus might not be transmitted by Aedes albopictus when temperature was below 18℃.The disseminated infection rate increased gradually with the increase of temperature.
