Headless HA of influenza H5N1virus constructs based on baculovirus expression system
10.3760/cma.j.issn.1003-9279.2012.03.006
- VernacularTitle:H5N1禽流感病毒headless HA基因在杆状病毒中的表达
- Author:
Li-Xia ZHANG
1
;
Zai-Jiang YU
;
Jian-Fang ZHOU
;
Kun QIN
;
Ying-Tian YU
;
Yue-Long SHU
Author Information
1. 延边大学
- Keywords:
Genes;
Influenza A virus;
Baculoviridae;
Expressed sequence tage
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(3):179-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct recombinant headless HA of avian influenza H5N1virus based on baculovirus expression system.Methods Headless HA gene of an avian H5N1virus,A/Hubei/1/2010 was cloned into pFastBac1vector.The recombinant shuttle vector,headless HA-Bac,was obtained by transforming pFastBac1headless HA into DH10bac cell and then the baculovirus bearing with headless HA gene,headlessHA-Bac,was achieved viaBac-to-Bac baculovirus expression system. The headless HA expression was detected in headless HA-Bac-infected Sf9 cells by Western Blot and HA assay.Results The headless HA has been efficiently expressed and displayed no reaction with turkey red blood cells.Conclusion The constructs provide fundamental work for future studies on universal influenza vaccine.
