Determination of six lactones in Mongolian medicine compound nutmeg-5 by HPLC
10.3969/j.issn.1006-2157.2016.01.011
- VernacularTitle:蒙药复方肉豆蔻五味中6个内酯的含量测定?
- Author:
Xiaoling ZHU
1
,
2
;
Haijie XU
;
Yu DONG
;
Zi TIAN
Author Information
1. 内蒙古医科大学药学院 内蒙古010110
2. 内蒙古国际蒙医医院药学部
- Keywords:
Mongolian medicine nutmeg-5;
santamarine;
costunolide;
dehydrocostuslactone;
alantola-tone;
isoalantolactone;
11,13-dihydroisoalantolantone;
HPLC
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;(1):55-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simultaneous quantitative determination method for six lactones (santamarine, costunolide, dehydrocostuslactone, alantolatone, isoalantolactone and 11,13-dihydroi-soalantolantone ) from nutmeg-5, a Mongolian medicine compound. Methods HPLC analysis was performed by an Elite C18 column(4. 6 mm × 250 mm,5 μm) at temperature 30℃ with gradient elution (acetonitrile and aqueous solution) at a flow rate of 0. 8 mL/min 204 nm. Results The six lactones were separated completedly by using the established HPLC method, and the regression equation of each lactone showed good linear correlation between concentration and peak area with the correlation coefficient more than 0. 999. Each average recovery rate with relative standard deviation (RSD) of six lactones was listed as the following:santamarine 102 . 54% ( RSD 1 . 02%) , costunolide 103. 35% ( RSD 0 . 82%) , dehydrocostuslactone 100 . 38% ( RSD 1 . 75%) , alantolatone 97 . 81% ( RSD 1 . 59%) , isoalantolactone 96. 65% (RSD 1. 98%), and 11,13-dihydroisoalantolantone 99. 64% (RSD 1. 68%). Conclusion The HPLC method was validated and found to be specific, linear, precise, and accurate. This chromato-graphic system can be readily used on a routine way for determination of lactones from nutmeg-5 .
