Research on recombinant human antibodies against hepatitis B surface antigen
10.3760/cma.j.issn.1003-9279.2012.03.004
- VernacularTitle:人源抗乙肝表面抗原基因工程抗体的研究
- Author:
Yang LIU
1
;
Li-Na SUN
;
Chuan LI
;
Fu-Shun ZHANG
;
Mi-Fang LIANG
;
De-Xin LI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hepatitis B surface antigens;
Bacteriophages;
Antibodies,viral;
Immunoglobulin G
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(3):172-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain recombinant human anti-HBsAg genetic engineering antibodies from volunteer-derived antibody phage library.Methods A combinatorial human Fab library against HBsAg was constructed with antibody genes harvested from peripheral blood of HBsAb + volunteers after HBsAg booster immunization.The library was panned with phage display and selected by purified HBsAg.After that the selected antibodies were converted to full human IgG antibody with recombinant baculovirus/insect cell system and mammal cell system.Results 20 different human Fab antibodies specific for HBsAg were obtained by ELISA and determination of subtype and subclass of antibodies after gene sequencing.Six specific anti-HBsAg human Fab antibodies were converted to full human IgG antibody.Competitive ELISA showed three of the six full human IgG antibodies had different epitopes against HBsAg.Conclusion Three monoclonal antibodies with different epitopes against HBsAg were panned and obtained successfully and laid the foundation for the therapeutic antibodies and new vaccines.
