Indirect competitive enzyme-linked immunosorbent assay for determina-tion of baicalin in Gegen Qinlian Tablet produced by seven manufactur-ers
10.3969/j.issn.1006-2157.2016.01.010
- VernacularTitle:间接竞争ELISA法测定不同厂家葛根芩连片中黄芩苷的含量?
- Author:
Shuchen LIU
1
;
Baoping QU
;
Hui KONG
;
Wenchao SHAN
;
Jinjun CHENG
;
Wenhao ZENG
;
Huihua QU
;
Qingguo WANG
;
Yan ZHAO
Author Information
1. 北京中医药大学基础医学院 北京100029
- Keywords:
indirect competitive enzyme-linked immunosorbent assay method;
Gegen Qinlian tablet;
ba-icalin;
content determination
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the contents of baicalin in Gegen Qinlian Tablet produced by seven manufacturers, a Chinese patent drug used widely in acute gastroenteritis, virus diarrhea, and bacillary dysentery, by using indirect competitive enzyme-linked immunosorbent assay ( ic-ELISA ) . Methods The ic-ELISA method was established by using anti-baicalin monoclonal antibody ( anti-BA MAb) , in which the linear relation, accuracy and the recovery rate were measured. Based on the ic-ELISA method, the contents of baicalin in Gegen Qinlian Tablet from different manufacturers were deter-mined. Results The linear range of the ic-ELISA method established was from 16. 157 to 1 280 mg/L, the relative standard deviation (RSD) was≤5. 1% in the intra-assay, and≤9. 0% in the inter-assay, with the average recovery rate of 107 . 1% ( RSD=4 . 9%) . The baicalin contents in Gegen Qinlian Tab-let from seven manufacturers ranged from 4. 45 to 13. 41 mg per tablet. Conclusion The ic-ELISA method for the baicalin determination was convenient, accurate, and repeatable, which could be applied to the quality control of traditional Chinese herbal compound containing baicalin.
