Effects of human gut microbiota in vitro on transformation of the total tannins content of Phyllanthus emblical L
10.3969/j.issn.1006-2157.2016.01.009
- VernacularTitle:人肠内菌对藏药余甘子鞣质部位代谢的研究
- Author:
Guanghui YANG
1
;
Lingfang WU
;
Xiaoxue ZHANG
;
Dan ZHU
;
Yanyan SHAO
;
Xin MAO
;
Wenyi LIANG
;
Wenjing CHEN
;
Renbing SHI
;
Lanzhen ZHANG
Author Information
1. 北京中医药大学中药学院 北京100102
- Keywords:
Phyllanthus emblica L.;
total tannins;
human gut microbiota;
metabolism
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study in vitro the effect of human intestinal bacteria on the transformation of to-tal tannins extracted from emblic leafflower ( Yuganzi, Phyllanthus emblica L. ) , a usually used Tibetan herbal medicine. Methods Based on the earlier stage work of the extraction of total tannins from Phyl-lanthus emblica L. , activated human gut microbiota was anaerobically incubated in the culture medium GAM containing the total tannins at 37℃ for 72 hours. After centrifugation and protein precipitation, the characteristics of the metabolites were determined by using HPLC/MSn and the contents of each compo-nent determined were measured by using HPLC-UV, on which the time-content curve was drawn. Results Total 13 components were determined, including four metabolites ( pyrogallic acid, methyl-de-rivative of gallic, urolithin B, and urolithin A) , four prototype components ( gallic acid, corilagin, gal-loyl glucose, and digalloyl glucose) and five components to be determined. The accumulated contents of gallic acid and ellagic acid were the highest at Hour 12 , 10-fold higher than that of the beginning; the accumulated content of corilagin was the highest at Hour 6 with twice higher of the beginning. Conclusion After the total tannins from Phyllanthus emblica L. were transformed by human gut microbi-ota in vitro, certain bioactive prototype components remained and antitumor components were found.
