Cross sectional study of genotype drug-resistance mutation in HIV infected individuals in Henan
10.3760/cma.j.issn.1003-9279.2012.03.002
- VernacularTitle:河南省某市HIV感染者基因型耐药横断面调查
- Author:
Xiao YAN
1
;
Jing MA
;
Xiao-Mei ZHANG
;
Zhan-Jun CHANG
;
Jian-Xun LIU
;
Lin LAN
;
Lan-Ying XU
;
Sheng-Yong ZHANG
;
Jian-Min BAI
;
Jian-Hua QIAN
;
Xiao-Guang ZHANG
;
Yi ZENG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
HIV;
Genes;
Cross sectional studies;
Drug resisitance,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(3):165-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find the prevalence of genotype drug-resistance mutation in HIV/AIDS infected individuals in Henan.Methods Totally 150 plasma samples were collected for BNA extraction from HIV/AIDS infected individuals in Henan in 2011.The HIV-1pol geue region was amplified by the methods of reverse transcription and nested-PCR.The phylogenetic tree was constructed based on DNA sequences,and HIV genotype drug-resistance mutation was analyzed.Results Thirty-four of 150 plasma samples were successfully sequenced (9 in untreated group and 25 in antiretroviral therapy group).HIV-1clade B' was major genetic subtype,which accounted for 94.1％ respectively.The percentage of HIV genotype drugresistance samples in sequenced samples was 58.8％ (5.9％ in untreated group and 52.9％ in antiretroviral therapy group).Conclusion The samples with genotypic resistance in antiretroviral therapy group showed resistance to RT inhibitors incoordinately and multidrug resistance.Monitoring and management system of drug resistance should be improved.Treatment should be tried to reduce the generation of HIV drugresistance strains.And adjustment of the drug scheme should be in time.
