Effects of chrysophanol on glutamate metabolism of primarily cultured strocytes in mice
10.3969/j.issn.1006-2157.2016.01.007
- VernacularTitle:大黄酚对原代培养小鼠星形胶质细胞谷氨酸代谢的影响?
- Author:
Zhanxia XUE
1
;
Yongshan GAO
;
Haixia WU
;
Junrong HAO
;
Lixia SHEN
Author Information
1. 河北北方学院药学系 河北075000
- Keywords:
chrysophanol;
astrocyte;
glutamate/aspartate transporter;
glutamate acid transporter 1;
glu-tathione peroxidase;
glutamine synthetase;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;(1):35-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of different concentration of chrysophanol on the activity and glutamate metabolism of primarily cultured astrocytes in vitro. Methods Primarily cultured mice astro-cytes were treated with chrysophanol at concentration of 10 , 20 , 40 , 60 , 80 , 100 , 200 , and 250 mg/L ( chry 1~8 group) for 1 d, 2 d, and 3 d. The cell activities were measured by MTT method, mRNA ex-pressions of glutamate/aspartate transporter (GLAST) and glutamate acid transporter 1(GLT-1) were de-tected by using RT-PCR, and the activities of glutamine synthetase ( GS ) and glutathione peroxidase ( GSH-Px ) were also determined. Results Time-dependence and concentration-dependence of the effects of chrysophanol showed on the following: activity of astrocytes inhibited, mRNA expressions of GLAST and GLT-1 upregulated, and the activities of GSH-Px and GS increased. However, the effect of chrysophanol on extra-cellular concentration of glutamate didn’ t depend on the number or activity of the cells. Conclusion Chrysophanol inhibited the activity of astrocytes in mice, and its effect on glutamate metabolism was concentration-and time-dependent.
