Influences of 11-ethoxyviburtinal of Valeriana jatamansi Jones on the ex-pression of enterochromaffin cells and mast cells in rats with irritable bowel syndrome
10.3969/j.issn.1006-2157.2016.01.004
- VernacularTitle:蜘蛛香成分11-ethoxyviburtinal对肠易激综合征大鼠肠嗜铬细胞及肥大细胞表达的影响?
- Author:
Congrui XIAO
1
;
Siyu TAO
;
Xingli YAN
;
Zengping GAO
;
Ruirui SHI
;
Juan WANG
;
Jing WANG
Author Information
1. 北京中医药大学中药学院 北京100102
- Keywords:
Valeriana jatamansi Jones;
11-ethoxyviburtinal;
irritable bowel syndrome;
enterochromaffin cell;
mast cell;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;(1):21-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 11-ethoxyviburtinal of Valeriana jatamansi Jones on the expression of enterochromaffin cells ( EC) and mast cells ( MC) in rats with irritable bowel syndrome ( IBS) . Methods The IBS rat model was induced by chronic unpredictable stress with isolation. Male SD rats were randomly divided into six groups:control group,model group, pinaverium group at the dose of 25 mg/kg,and high-, mid-and low-dose of 11-ethoxyviburtinal group at dose of 1. 2, 0. 6, and 0. 3 mg/kg respectively. The number of 5-HT positive immunoreactive colonic EC was detected by the method of immunohistochemistry and that of colonic MC was measured with toluidine blue staining. Results Compared with control group, the number of 5-HT positive immunoreactive colonic EC and MC increased significantly in model group ( P <0 . 05 ) . The high dose of 11-ethoxyviburtinal could significantly de-crease the number of colonic EC and MC ( P<0 . 05 ) . Conclusion The 11-ethoxyviburtinal of Valeri-ana jatamansi Jones could significantly reduce the number of 5-HT positive immunoreactive 5-HT positive immunoreactive EC and MC in colon tissue of rats with IBS.
