Establish and preliminary application of loop mediated isothermal amplification (LAMP) technique in detection for human bocavirus

VernacularTitle:人博卡病毒LAMP检测方法的建立及初步应用
Author: Xiang WANG ¹ ; Xin-Gang XU ; Qian ZHANG ; Fang ZHANG ; Wu-Mei YUAN ; Fen-Lian MA ; Peng-Hao XUE ; Li-Shu ZHENG
Author Information

1. 西北农林科技大学
Keywords: Parvoviridae; Genes; Nucleic acid amplification techniques
From: Chinese Journal of Experimental and Clinical Virology 2010;24(4):308-310
CountryChina
Language:Chinese
Abstract: Objective To establish the methods of LAMP assay in human bocavirus(HBoV) and use to detect clinical samples. Methods Establish LAMP assay for HBoV by using primer explorer V4 (LAMP primer design software) on line to design LAMP primer for sequence NP1 of HBoV. Evaluate its sensitivity and specificity. Results Comparing with traditional PCR, LAMP shows good specificity and higher sensitivity until 10 copy. By LAMP assay we get 5 positive samples in 76 clinical swabs from the children with respiratory infections. Conclusion LAMP assay for detection of HBoV is sensitive, efficient,rapid and simple. It is a new way to detect HBoV in clinical test.