Application of immuno-rotary biosensor based on F0F1 -ATPase in chromatophores for detecting HIV-1 P24

VernacularTitle:免疫旋转生物传感器高灵敏检测HIV-1 P24抗原方法的研究
Author: Yun-Hu SANG ¹ ; Xiao-Mei ZHANG ; Xiao-Guang ZHANG ; Jing MA ; Xue-Ren LI ; Jia-Chang YUE ; Yi ZENG
Author Information

1. 北京工业大学
Keywords: HIV; Immuno-rotary biosensor; ATP synthetase complexes
From: Chinese Journal of Experimental and Clinical Virology 2010;24(4):305-307
CountryChina
Language:Chinese
Abstract: Objective To build a high sensitive method to detect HIV-1 P24 antigen quantitatively by Immuno-rotary Biosensor (IRB) based on F0F1-ATPase. Methods The immuno-rotary biosensor based on F0F1-ATPase in chromatophores for detecting HIV-1 P24 was assembled by label F0F1-ATPase chromatophores with the biotin-labled HIV-1 P24 antibody through the streptavidin-biotin-β antibody and the pH sensitivity of fluorescence F1300. Then it's sensitivity and specificity was tested by the prokaryotic expressed HIV-1 P24 antigen. Results Immuno-rotary biosensor based on F0F1-ATPase in chromatophores can detect P24 antigen sensitivily, the lowest level that could be detected was 0.1 fg/ml. Conclusion IRBbased system was successfully assembled and used for the detection of P24 antigen, the rapid and sensitive technique will be useful for detecting HIV-1 P24 antigen quantitatively.