Simultaneous and rapid detection of influenza viruses A and B by multiplex real-time quantitative PCR
10.3760/cma.j.issn.1003-9279.2010.04.019
- VernacularTitle:双重荧光定量RT-PCR在快速检测A、B型流感病毒上的应用
- Author:
Jian-Kang HAN
1
;
De-Shun XU
;
Lei JI
;
Xiao-Fang WU
Author Information
1. 浙江省湖州市疾病预防控制中心
- Keywords:
Orthomyxoviridae;
Sequence analysis;
Polymerase china reaction
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(4):295-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a TaqMan-based multiplex real-time PCR assay for detection of influenza viruses A and B, and the constructed method was primarily applied to clinical samples test.Methods The specific primers and probes were designed in the conserved region of the M and HA gene of influenza viruses A and B, respectively. The reaction conditions were optimized and the sensitivity, specificity and the stab ility of the assay were evaluated. The clinical specimens collected from the patients were detected by this assay. Results For specific detecting the influenza A and B viruses,the detection limits of the assay were 0.1TCID50and 0.01 TCID50. The viral RNA was directly detected from the clinical specimens by this assay. Only three hours were needed for viral RNA extraction and real-time PCR amplification.Conclusion This assay is a rapid, sensitive and specific one for simultaneous detection of influenza viruses A and B.
