Chemotherapy-induced premature ovarian failure rats treated by TCM therapy of tonifying kidney-yin with bone marrow mesenchymal stem cells transplantation
10.3969/j.issn.1006-2157.2015.11.004
- VernacularTitle:补肾养阴法协同干细胞移植治疗化疗后卵巢早衰的实验研究
- Author:
Jiajia QIN
1
;
Qian WU
;
Jing YANG
;
Jiali LIANG
Author Information
1. 暨南大学医学院 广东 510632
- Keywords:
premature ovarian failure;
bone marrow mesenchymal stem cell;
TCM therapy of tonifying kidney-yin;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(11):735-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of Zuogui pill (ZGP),a traditional Chinese medicine formulated by TCMtherapy of tonifying kidney-yin,with the therapy of bone marrow mesenchymal stem cells (BMSC ) transplantation in premature ovarian failure (POF ) induced by chemotherapy. Methods The rat model of POF was established by intraperitoneal injection of cyclophosphamide (cytoxan,CTX ) for consecutive 14 d.All the PDF rats were divided into model group,BMSC transplantation (BMSCT)group and BMSCT combined with ZGP (BMSCT +ZGP)group,and the healthy young rats were included in control group.The levels of estradiol (E2 ),follicle-stimulating hormone (FSH)in peripheral blood were determined by using ELISA ,the ovarian granulosa cell apoptosis were tested by Tunel assay,and the mRNA expressions of Bcl-2 Associated X (Bax)and B-cell lymphoma-2 (Bcl2)were examined by Western blot.Results After successful modeling,the level of E2 in peripheral blood in the model group was much lower than that in the control group,and the level of FSH in peripheral blood was much higher(both P <0.01).Compared with model group,the level of E2 increased gradually while FSH decreased guadually in both BMSCT group and BMSCT +ZGP group(P<0.01),and the changes of E2 and FSH were more obvious in BMSCT +ZGP group than those in BM-SCT group.Tunel showed that the number of cells decreased in both BMSCT group and BMSCT +ZGP group,compared with model group,but there were no significant difference between the two treatment groups.The mRNA expressions of Bax were lower in both treatment groups than that of model group,and the significant decreasing was found in BMSCT +ZGP group compared with model group(P <0.05).The mRNA expressions of Bcl2 increased significantly after BMSCT and ZGP treatment compared with model group(P <0.01).Conclusion BMSCT combined with ZGP could improve ovarian function in POF rats induced by chemotherapy,and the effect was better than that of BMSCT only.
