Study of the expression of complement regulatory protein D59 on T cells in HIV infected patients
10.3760/cma.j.issn.1003-9279.2010.04.009
- VernacularTitle:补体调节蛋白CD59在HIV感染者外周血T细胞上的表达研究
- Author:
Pei-Ze ZHANG
1
;
Hui WANG
;
Ming-Xia ZHANG
;
Qiao-Li PENG
;
Gui-Ying LI
;
Shui-Teng LIU
;
Yan LIU
;
Xin-Chun CHEN
;
Tai-Sheng LI
;
Bo-Ping ZHOU
Author Information
1. 深圳市第三人民医院
- Keywords:
HIV-1;
Antigens,CD59;
CD4-positive T-lymphocytes;
Antiviral agemts
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(4):263-266
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe how complement regulatory protein CD59 expression on T cells in HIV infected patients and discuss the meaning of how highly active antiretroviral therapy(HARRT) affect CD59 expression. Methods 48 HIV infected patients and 14 healthy donors were performed in this study.Patients were divided into Naive group and On-HARRT group according to HARRT or not. The peripheral blood samples were collected and cell surface cytokines were stained, and then were evaluated with the BD FACS Canto flow cytometry, after that, the expression of CD59 on CD4+T, CD8+T and memory CD45ROCD4+T cells were analyzed, and HIVRNA were detected with PCR, then compare the results between groups. Results Compared with healthy donor, the expression of CD59 on T cells in HIV infected patients are significantly higher(P＜0.05), most of that expressed on CD45ROCD4+T cells(P＜0.05).Compared with Naive group, the CD59 expression on CD4+T cells in On-HARRT group decreased significantly but it is still higher while compared with healthy control(P＜0.05). CD59 expression on CD4+T cells is correlated with HIVRNA and CD4+T cells count(R2=0.2181, P=0.0247; R2=0.1586, P=0.0486). Conclusion HIV infection can cause CD59 expression increase on CD4+T cells and HARRT can decrease its expression. This increase may be related to HIV immune escape and CD4 +T cell function inhibition, and HARRT can partially reverse this immune disorder.
