The influence of the quantity of HBV-specific CTLs with therapeutic vaccine
10.3760/cma.j.issn.1003-9279.2010.04.006
- VernacularTitle:治疗用合成肽乙肝疫苗对CTLs数量的影响
- Author:
Fang-Fang LIU
1
;
Hong-Li XI
;
Yan-Yan YU
;
Chong-Wen SI
;
Zheng ZENG
Author Information
1. 北京大学第一医院
- Keywords:
Hepatitis B virus;
Vaccines;
T-lymphosytes,cytotoxic;
Peptides
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(4):254-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of therapeutic vaccine on the quantity of HBV-specific cytotoxic T lymphocytes(CTLs), and investigate the relationship of HBV-specific CTLs quantity with HBV viral load. Methods Fifteen HLA-A2-positive chronic hepatitis B (CHB) patients were randomly divided into three groups: single vaccine group, control group and combination group. With MHC-I-peptide pentamers and multi-colorFACS, the number of HBV-specific CTLs was measured in baseline, treatment and follow-up 24 weeks; the correlation between the quantity of HBV-specific CTLs and HBV viral load was studied. The quantitation of HBV DNA was determined by Light Cycler fluorescent quantitative PCR.Results (1) There were no difference in the quantity of CTLs (0. 022% to 0. 028%) in baseline among three groups (P ＞ 0.05). (2) In both single vaccine group and combination group, the quantity of CTLs was higher than that of control group on during treatment and follow-up weeks ( P ＜ 0. 05 ). And there was no difference between single vaccine group and combination group on quantity of CTLs (P ＞ 0. 05). (3) HBVspecific CTLs level correlated negatively with HBV viral load among these three groups ( P ＜ 0. 05 ).Conclusion In this study, it showed that the quantity of HBV-specific CTLs was specific increased by using therapeutic vaccine (synthetic peptide), which may inhibit HBV replication.
