Effects of Yiqi Huoxue Jiedu Fang on gene expression of cytokines involving NF-κB signal pathway in collagen II-induced arthritic rats
10.3969/j.issn.1006-2157.2015.10.009
- VernacularTitle:益气活血解毒中药对胶原诱导性关节炎大鼠NF-κB信号通路相关因子表达的影响
- Author:
Bin DONG
1
;
Lixia LOU
;
Yi WEI
;
Jiuli ZHAO
;
Qiushuo JIN
;
Limin CHAI
Author Information
1. 北京中医药大学东直门医院 中医内科学教育部和北京市重点实验室 北京100700
- Keywords:
Yiqi Huoxue Jiedu Fang;
collagen II-induced arthritis;
inflammatory cytokines;
NF-κB sig-nal pathway;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;38(10):686-689,702,721
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the gene expressions of key cytokines involving NF-κB signaling pathway in collagen II-induced arthritic ( CIA ) rats treated with Yiqi Huoxue Jiedu Fang ( qi-tonifying blood-activating and toxin-removing formula) .Methods We used collagen II-induced arthritic ( CIA) rats by bovine II collagen as an animal model and Leflunomide as controls.CIA rats received treatment with Yiqi Huoxue Jiedu Fang.The levels of inflammatory cytokines in serum were measured with protein chip assay, and hematoxylin dyeing was used to observe the histopathological changes.The gene expressions of the key cytokines in NF-κB signal pathway were detected by real-time PCR.Results This formula inhibited the proliferation of synovial tissues, reduced inflammatory exudation and narrowed the range of fibroid necrosis.It also down-regulated inflammatory cytokines ( TNF, IL-1, IL-5, IL-6 and IL-10) in serum of CIA rats.The gene expressions of NF-κB and IKK decreased in CIA rats treated with this formula.Conclusion Yiqi Huoxue Jiedu Fang could regulate the expressions of inflammatory cytokines and the key factor of NF-κB signal pathway for inhibiting inflammatory reaction in injured tissues, restoring the joint injury.Those could be the key active mechanism of RA treated with this formula.
