Anti-allergy mechanism of fangfeng-wumei pair based on expressions of PAR-2

VernacularTitle:基于蛋白酶激活受体2表达研究药对防风-乌梅抗过敏的配伍机制
Author: Xianbo WU ¹ ; Haiyan ZHU ; Xiang LI ; Yugang XU ; Shenrui JIN ; Mingyi WANG
Author Information

1. 延边中医医院延吉市中医医院吉林133000
Keywords: Fangfeng; Wumei; medicinal pair; PAR-2
From: Journal of Beijing University of Traditional Chinese Medicine 2015;38(10):682-685
CountryChina
Language:Chinese
Abstract: Objective By observing the effects fangfeng-wumei pair on degranulation of mast cells and PAR-2 expression before and after the pairing to provide experimental basis for the research on its anti-allergy mechanism.Methods A mast cell degranulation mouse model was established by stimulating mouse mast cell line P815 with trysin in vitro, and the levels of histamine as well as PAR-2mRNA and protein expression of P815 cells affected by fangfeng-wumei pair were measured using RT-PCR, Western blotting and ELISA.Results Fangfeng-wumei pair in vitro significantly inhibited the secretion of histamine and the expression of PAR-2 by mast cells in comparison to cell model group ( P <0.05 or P<0.01 ) .Conclusion The results demonstrated that inhibition of degranulation of mast cells was significantly enhanced by fangfeng-wumei pair, and that the expression of PAR-2 by mast cells might be involved in the anti-allergy mechanism of fangfeng-wumei pair.