Effects of kidney-tonifying qi-regulating formula on time-course expression of bFGF in rat limbic system with cerebral ischemia
10.3969/j.issn.1006-2157.2015.10.006
- VernacularTitle:益肾调气中药对缺血性脑卒中后边缘系统碱性成纤维生长因子时相变化的影响
- Author:
Xiaoli LI
1
;
Qisheng TANG
;
Dongmei LI
;
Ge WANG
;
Xinke YANG
Author Information
1. 北京中医药大学第三附属医院 北京100029
- Keywords:
cerebral ischemia;
mental disorder;
limbic system;
bFGF;
Yishen Tiaoqi Formula;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;38(10):672-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the time-course expressions of the basic fibroblast growth factor ( bFGF) in limbic system of cerebral ischemic rats and explore the effects of Yishen Tiaoqi ( kidney-tonifying qi-regulating) Formula.Methods Male Wistar rats were randomly divided into normal group , sham-operation group, MCI group, treatment group and control group according to the scores of Open-Field test. The normal group received no intervention. The sham-operation group received sham operation, and intragastric administration of distilled water.Homogenous microthrombus were injected into internal carotid artery through the external carotid artery of those rats in other groups to duplicate MCI model first.The treatment group and control group received intragastric administration of Yishen Tiaoqi Formula (6.2g/kg) and Nimodipine (9.45mg/kg) respectively.The changes of limbic bFGF and intervention efficacy of Chinese medicinal were observed by using immunohistochemistry technique in the 1st Week,2nd Week and 4th Week.Results Immunohistochemistry test showed that positive cells of bFGF increased significantly in MCI group.The expression of bFGF was significantly higher in the first week than in other time points in MCI group, reached peak at day 7 and then decreased gradually.The positive cells of bFGF increased significantly in Chinese medicinal group compared with MCI group and normal group in the 2nd Week and 4th Week.Conclusion There are changes of limbic bFGF in rats after cerebrovascular disease, and Yishen Tiaoqi Formula can block mental disorder after stroke with improved vascular repair and regeneration.
