Complete genome analysis of a newly isolated enterovirus

VernacularTitle:新型肠道病毒分离株(BJ302)全基因组序列测定及分析
Author: Jun HOU ¹ ; Yan HU ; Hong-Hui SHENG ; Bing-Ke BAI ; Zhi-Jie WANG ; Pan-Yong MAO
Author Information

1. 解放军第302医院
Keywords: Enterovirus; Genome; Genes,structural,viral
From: Chinese Journal of Experimental and Clinical Virology 2008;22(2):110-112
CountryChina
Language:Chinese
Abstract: Objective To demonstrate molecular characterization of a newly isolated enterovirus.Methods Virus were isolated from patient with unknown-causing disease and tested by reverse transcription-polymerase chain reaction (RT-PCR) and 5' ,3'RACE (rapid amplification of eDNA ends, RACE), in an attempt to obtain the sequence of this newly isolated enterovirus. Results Sequence analysis showed that this newly isolated enterovirus shared 83 %-94% nucleotide identity and 91%-100% amino acid identity with enterovirus 89.Phylogenetic analysis indicated that it was probably a new subtype of enterovirus 89. Conclusion This newly isolated enterovirus in the stool specimen from patient has the same serotype with enterovirus 89, but it was probably a new subtype of enterovirus 89.