Characteristic of intestinal absorption of euphorbia factor L1 by rats single pass intestinal perfusion moder in situ
10.3969/j.issn.1006-2157.2015.09.010
- VernacularTitle:在体单向肠灌流模型研究千金子甾醇在大鼠肠吸收特性
- Author:
Xiuting ZHANG
1
;
Yingzi WANG
;
Shaojing LI
;
Feipeng DUAN
;
Qing WANG
;
Chunni ZHANG
;
Fengying LI
;
Wenhua LI
;
Shengxiu LUO
Author Information
1. 北京中医药大学中药学院 北京100102
- Keywords:
single-pass intestinal perfusion;
euphorbia factor L1;
P-glycoprotein;
multidrug resistance-as-sociated protein;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(9):624-627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the characteristic of absorption of euphorbia factor L1 in intestine of rats, and to observe the effects of P-glycoprotein(P-gp)and multidrug resistance-associated protein(MRP2)on intestinal absorption of euphorbia factor L1 .Methods The contents of euphorbia factor L1 of intestinal perfusion fluid of duodenum, jejunum, ileum and colon in the rats in situ single-pass intestinal perfusion model were determined by using HPLC.The drug absorption rate constant( Ka ) and the apparent absorp-tion coefficient( Papp ) in four intestinal regions were calculated.Results Ka and Papp of euphorbia factor L1 at colon were the highest of the whole rat intestine.Significant increase of Ka and Papp showed at rat colon when co-perfused with verapamil hydrochloride, by contrast, decrease of Ka and Papp found when co-perfused with indomethacin.Conclusion We inferred that verapamil hydrochloride be the substrate of P-gp and that indomethacin be not the substrate of MRP2 .
