Effects of serum containing modified Shengmai liquid on migration and adhesion of non small cell lung cancer cells
10.3969/j.issn.1006-2157.2015.09.008
- VernacularTitle:加味生脉饮含药血清对非小细胞肺癌细胞迁移及黏附的影响
- Author:
Hui CHEN
1
;
Miao JIANG
;
Lili XIN
;
Jinglian QU
;
Muge XU
;
Yinxu SU
;
Jiening GONG
Author Information
1. 南京中医药大学 江苏210046
- Keywords:
serum containing modified Shengmai liquid;
non small cell lung cancer;
migration;
adhe-sion;
cell cultured
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(9):606-610
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects and mechanism of serum containing modified Shengmai liquid ( MSL) on migration and adhesion of non small cell lung cancer ( NSCLC) cells.Methods Rabbit ser-um containing MSL was prepared by intragastric administration ( i.g.) with MSL at the dose of 18 g/kg and control serum was prepared by i.g.normal saline.NSCLC cells were cultured, and those in logarith-mic growth phase collected were divided into four groups:10%control serum(CS) group, 2.5%, 5%, 10%MSL serum group.Migration cells were detected by using Transwell invasion assay, and adhension rate was measured by using matrigel adhensive assay.The expressions of N-cad and vimentin were de-termined by RT-PCR.Results The number of migration cells decreased as the concentration of serum containg MSL increased:migration rate of 5%MSL group was 25.64%and that of 10%MSL group was 13.31%(P<0.01).As the concentration of serum containing MSL increased and the duration of treat-ment prolonged, heterogeneous adhesion rate decreased and was negatively correlated with serum concen-tration and duration;while the gene expressions of N-cad and vimentin decreased in dose-and time-de-pendent manner.Conclusion The mechanism of inhabitation migration and reduction heterogeneity ad-hension of MSL on NSCLC cells would be related to the epithelial-mesenchymal transition.
