Effect of the method of clearing heat and resolving phlegm on the airway mucus hypersecretion of the AECOPD rat model with phlegm-heat syn-drome
10.3969/j.issn.1006-2157.2015.09.005
- VernacularTitle:清热化痰法对慢性阻塞性肺疾病急性加重期痰热证大鼠气道黏液高分泌的影响
- Author:
Jie LI
1
;
Qi WANG
;
Lishan ZHANG
;
Zeji QIU
;
Jun WU
;
Linyang WANG
Author Information
1. 北京中医药大学东直门医院 北京100700
- Keywords:
method of clearing heat and resolving phlegm;
AECOPD;
phlegm-heat syndrome;
airway mucus hypersecretion;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(9):601-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Louqin Zhisou decoction ( LQZSD) , a Chinese herbal medi-cine compound formulated by the TCM method of clearing heat and resolving phlegm, on the airway mu-cus hypersecretion of the AECOPD rat model, so as to improve the transportation of antibiotics in lung tis-sue .Methods Altogether 50 Wistar male rats were randomly divided into normal group , model group , ambroxol group , Erchen decoction ( ECD) group, Louqin Zhishou decoction( LQZSD) group ( each n=10) .The rat model of AECOPD with phlegm-heat syndrome was established by passive cigarette smok-ing,intratracheal instillation of lipopolysacchricle ( LPY) and intranose instillation of staphylococcus au-reus combined with wind-heat stimulation.After modeling, ambroxol group was intraperitoneally injected ambroxol at dose of 6 mg/kg per day, ECD group and LQZSD group were intragastrically administered ECD and LQZSD at dose of 3.33 g/kg per day respectively.The treatment duration was seven days.The secretion of acidic mucin in goblet cells was observed by using AB-PAS staining.The goblet cells in each visual field were counted and the proportion of goblet cells to epithelial cells was calculated .The contents of serum neutrophil elastase ( NE) and MUC5AC in bronchoalveolar lavage fluid ( BALF) were detected by using ELISA assay.Results Compared with model group, the secretion of acidic mucin and the number of goblet cells significantly decreased in each treatment group(P<0.05).Furthermore, serum NE and MUC5AC both decreased in LQZSD group significantly(P<0.05), while there were no statisti-cal differences in the other treatment groups.Conclusion The potential mechanism of LQZSD improving the transportation of antibiotics would be related to reduction the secretion of acidic mucin and the number of goblet cells, as well as decrease the contents of serum NE and MUC5AC in BALF;as the result, the reduction of the secretion of mucus in airway led to clearance the airway obstruction and promotion the forward diffusion of antibiotics in rat’ s lung tissue.
