Comparison of two chromatographic methods for the determination of sugars in Danshen Injection (lyophilized powder)
10.3969/j.issn.1006-2157.2015.08.011
- VernacularTitle:注射用丹参（冻干）中糖类成分的两种色谱测定方法比较
- Author:
Jing LIU
1
;
Chun-Xia HAN
;
Yan JIANG
;
Gang-Li WANG
;
Rui-Chao LIN
;
Zhong DAI
;
Shuang-Cheng MA
Author Information
1. 中国食品药品检定研究院中药所 北京 100050
- Keywords:
Danshen Injection (lyophilized powder);
sugars;
evaporative laser light detection;
ion chro-matography
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(8):556-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare two chromatographic methods and then to select an accurate,conven-ient and rapid method for the determination of sugars in Danshen Injection (lyophilized powder). Methods Firstly,HPLC -ELSD method was applied on an Alltech PrevailTM Carbohydrate ES (4.6 mm ×250 mm,5 μm)column,and acetonitrile-water (76∶24)was used as the mobile phase on an isocrat-ic elution with ELSD as the detector.Apart from that,IC method was adopted on a CarboPac PA20 (150 mm ×3 mm,S /N 003654 )anion exchange column with the CarboPac PA20 (50 ×3 mm,S /N 003405)column as the guard column,and sodium hydrate solution (7 mmol /L)was used as eluent with the pulsed amperometric detector.To sum up,HPLC-ELSD and IC methods described above were ap-plied to determine the sugars in Danshen Injection (lyophilized powder)respectively.Results Under the chromatographic conditions mentioned above,HPLC-ELSD method could simultaneously determine the contents of fructose and glucose.Linear relationship,precision,repeatability and recovery rate of the method were all qualified;IC method could simultaneously determine arabinose,galactose,glucose,su-crose,mannose as well as fructose,and the method proved to be accurate and reliable.The determina-tion results of fructose and glucose by both methods were basically the same.Conclusion IC method seems to be a better choice to determine the monosaccharides and disaccharide in Danshen Injection (ly-ophilized powder).
