Effects of Mongolian formula Shudage-4 on gastric ulcer
10.3969/j.issn.1006-2157.2015.08.004
- VernacularTitle:蒙药述达格－4抗小鼠胃溃疡的作用研究
- Author:
Sha-Sha XIN
1
;
Yang YANG
;
Pu BAI
;
Yu DONG
Author Information
1. 内蒙古医科大学药学院 内蒙古 010059
- Keywords:
Shudage-4;
ulcer index;
nitric oxide;
malonydialdehyde;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(8):524-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Mongolian formula Shudage-4 on alcohol-induced gastric ulcer in mice,and to discuss its protective mechanism for gastric mucosa.Methods Kunming mice were randomly divided into Shudage-4 groups of low-dose,medium-dose,and high-dose,Anweiyang (patent Chinese medicine for peptic ulcer)group,cimetidine group,model group,and normal group. Ethanol was used to induce gastric mucosal injury for all the groups except the normal group.Ulcer index and ulcer inhibition rate were measured.Concentration of nitric oxide (NO)and malonydialdehyde (MDA)were detected by using ELISA.Liver tissue sections were examined under a light microscope. Stomach tissues were stained using Hematoxylin-eosin (H-E)staining and histopathological changes were observed via light microscopy.Results NO concentration increased significantly and MDA decreased significantly in the low-,medium-,and high-dose Mongolian Medicine Shudage-4 (P <0.05)groups,as compared with model group.NO concentration in the positive group was higher than the low-dose group, but lower than the medium-dose group and high-dose group,whereas MDA level in the positive group was lower than the low-dose group,but higher than the medium-dose group and high-dose group.Since the medium-dose group was shown to be the highest in NO concentration and lowest in MDA levels among the high-,medium-and low-dose Shudage-4 groups,the medium-dose group was chosen to be compared with the positive group.However,no significant difference was present between them (P >0.05 ).Mean-while,inflammatory responses were alleviated,as compared with the model group,in the high-,medium-and low-dose groups,especially in the latter two groups,with drastically reduced inflammatory cells and improved pathological conditions.Conclusion Mongolian Medicine Shudage-4 could significantly reduce the ethanol-induced gastric mucosal injury in mice,and may play a protective role.
