Effects of electroacupuncture intervention at different time-points on the histological structure,function and level of nitric oxide of the liver in sim-ulated weightlessness rats
10.3969/j.issn.1006-2157.2015.07.011
- VernacularTitle:不同时机介入电针对模拟失重大鼠肝脏组织结构、功能和一氧化氮水平的影响
- Author:
Yan SONG
1
;
Guo-Zhen ZHAO
;
De-Sheng WANG
;
Bai-Xiao ZHAO
;
Bo JI
;
He ZHANG
;
Ying-Qiu MAO
;
Ping ZHANG
;
Yong-Si XU
;
Ya-Li LIU
;
Ya-Wen LU
;
Jian DAI
;
Ying-Hui LI
Author Information
1. 北京中医药大学 北京 100029
- Keywords:
acupuncture;
simulated weightlessness;
liver injury;
ALT;
NO;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(7):481-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of needling on the structure,function and level of nitric oxide (NO)of the liver in rats with simulated weightlessness,so as to explore the protective mechanism of acu-puncture on liver injury.Methods Twenty male clean grade Wistar rats were randomly divided into nor-mal group,model group,pretreatment group and treatment group(each n =5).The rats of normal group received no treatment,and those of other three groups were tail -suspended for 4 weeks to establish weightlessness model.Electroacupuncture at the points of Shenshu (BL23),Pishu (BL20)and Sanyin-jiao (SP60)once daily for 30 min was applied one week ahead of tail-suspension in pre-treatment group, while in treatment group the needling treatment applied at the same three points once every other day for 14 d was carried out at the day when the rats was tail -suspended.The changes of hepatic histological structure were observed,and the activity of alanine aminotransferase(ALT)in serum and the content of NO in liver were measured.Results The hepatic cell degeneration were observed around the central vein in model group;the degree of degeneration relieved in both electroacupuncture treatment groups.Further-more,the curative effect of the pre-treatment group was better than the treatment group.Compared with normal group,the activity of ALT in serum and the content of NO in liver in model group increased signif-icantly (P <0.05,P <0.01).Compared with model group,the activity of ALT in serum and the con-tent of NO in liver in the pre-treatment group significantly reduced(P <0.05),however,those in treat-ment group had no obvious difference.Compared with pre-treatment group,the activity of ALT tended to increase and the content of NO increased significantly(P <0.01)in the acupuncture group.Conclusion The effects of relieving hepatic injury and dysfunction induced by weightlessness,which potentially was related to the content of NO,in the pre-treatment group were superior to those in the treatment group.
