Mesaconitine extracted from Radix Aconiti Lateralis Preparata Aconite in-hibited proliferation and induced apoptosis of human erythroleukemia (K562)cell line
10.3969/j.issn.1006-2157.2015.07.009
- VernacularTitle:附子中的新乌头碱对 K562细胞增殖和凋亡的影响
- Author:
Xin GUAN
1
;
Tong-Hua YANG
;
Ai-Ling ZHANG
;
Mian-Cheng SU
;
Ren-Bin ZHAO
;
Chao-Ran ZHANG
;
Li-Li HE
Author Information
1. 云南省第一人民医院血液科 云南 650032
- Keywords:
mesaconitine;
K562 cells;
cell proliferation;
cell cycle;
apoptosis
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(7):467-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological effect of mesaconitine extracted from Fuzi (Radix Aconiti Lateralis Preparata Aconite)on human erythroleukemia (K562)cells lines.Methods The contents of mesaconitine and benzoylmesaconine were determined by using HPLC.After K562 cells were treated with mesaconitine standard substance,the leukemia cell morphology was observed under an electron micro-scope microscope.And the cell cycle,apoptosis of leukemic cells were measured by flow cytometry.Re-sults Mesaconitine of 25 ~50 mg/L significantly inhibited K562 cells proliferation in dose -and time -dependent manner(P <0.05).Apoptosis was induced by mesaconitine at the dose of 25 ~50 mg/L(P <0.05).The ratio of cells at S phase increased and that of cells at G1 phase decreased(P <0.05).Con-clusion Mesaconitine extracted from Fuzi inhibited proliferation and induced apoptosis of K562 cell line.
