Typing of enteric adenoviruses in feces of infants with diarrhea
10.3760/cma.j.issn.1003-9279.2000.03.020
- VernacularTitle:婴幼儿腹泻物中肠道腺病毒的检测
- Author:
Yaqing HE
1
;
Hong YANG
Author Information
1. Shenzhen HeaIth and Anti - e[pidemic Station
- Keywords:
Adenoviruses,human;
Restriction edonuclease;
Diarrhea
- From:
Chinese Journal of Experimental and Clinical Virology
2000;14(3):278-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study of adenoirus Methods 114 fecal samples from dianhea infanes were teseea by PCR.The amplified procucts were analyzed by taqIand Rsa I endonuclease.Results The positive rate of adenovirus DNA in fecal samples was 14%(16/114). The amplified products products started with Taq I endonudease to identify the type of adenovirus,11 cleaved by Taq I yielded two fragments of 191 bp and 110 bp,the positive rate of entric adenovinus was 9.65% (11/114). Followed by Rsa I cleavage,two 45 bp fragments (Ad40), the positive rate of Ad40 was 1.75% (2/114), nine amplified products yielded 211 bp and 90 bp fragments (Ad41),the pos-itive rate of Ad41 was 7.89%(9/114).Conclusion The positive rate's of the adenovirus types 40 and 41 were 9.65% in feces of infants with diarrhea, and that of the other types of adenovirus was 4.35%.
