Mechanism of invigorating kidney and activating blood recipe for the treatment of diabetic erectile dysfunction in rats
10.3969/j.issn.1006-2157.2015.07.008
- VernacularTitle:补肾活血方治疗大鼠糖尿病性勃起功能障碍的机理
- Author:
Zeng-Bao YUE
1
;
Wen-Jun MA
;
Mao QIN
;
Bao-Xing LIU
;
Ming-Hui KE
;
Chuan-Hang WANG
Author Information
1. 中日友好医院 北京 100029
- Keywords:
diabetes millitus;
erectile dysfunction;
invigorating kidney and activating blood;
pAKT/AKT pathway;
cell apoptosis;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(7):462-466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the therapeutic effect and mechanism of invigorating kidney and activa-ting blood(IKAB)recipe,a Chinese herb compound,on diabetic erectile dysfunction (DED)in rats. Methods 40 male SD rats were randomly divided into four groups (each n =10):normal group,model group,low-dose IKAB group (low-dose group)and high-dose IKAB group(high-dose group).The rat model with 2 type of diabet mellitus was induced by intraperitoneal injection of streptozotocin (STZ). Normal group and model group were intragastrically administered (ig.)with normal saline,low-dose group was ig.3 g/kg of IKAB and high-dose group was ig.6 g/kg IKAB for consecutive 8 weeks.The penis viscera index was measured;the intracavernous pressure (ICP)were detected by Biopack electro-physiological instrument;the expressions of pAKT and AKT were detectd by Western blotting;cell apop-tosis of corpus cavernosum smooth muscle were measured by using TUNEL method and the pathological changes in penile tissue were observed by using Masson trichrome staining.Results Compared with model group,the penis viscera index of both IKAB groups had no statistical differences (P >0.05);the expressions of pAKT and AKT increased significantly(P <0.05);although the ratio of cell apoptosis of corpus cavernosum smooth muscle decreased,the statistical differences were not shown (P >0.05).Fur-thermore,ICP and ICP/MAP increased markedly in high-dose group and the collagen fiber proportion de-creased in low -dose group compared with model group (P <0.05).Conclusion The mechanism of in-vigorating kidney and activating blood recipe increasing ICP in rat model with DMis related to increasing the expression of pAKT,inhibiting cell apoptosis of corpus cavernosum smooth muscle and reducing colla-gen fibers formation in penis.
