Effects of liver-soothing heat-clearing and spleen-fortifying method on the ex-pressions of cerebral bax and bcl-2 in rats with generalized anxiety disease
10.3969/j.issn.1006-2157.2015.06.005
- VernacularTitle:疏肝清热健脾法对广泛性焦虑大鼠脑内bax、bcl-2表达的影响?
- Author:
Ning DONG
1
;
Qi-Sheng TANG
;
Rui-Zhen ZHAO
;
Bin LUO
;
Wen-Long WANG
Author Information
1. 北京中医药大学 北京100029
- Keywords:
liver-soothing heat-clearing and spleen-fortifying method;
generalized anxiety disease;
Pa-pez’ s circuit;
bcl-2;
bax;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2015;(6):383-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of bax and bcl-2 in cerebral Papez’ s circuit in rat with generalized Anxiety Disease ( GAD) . Methods A total of 40 male Wistar rats were divided into normal group, model group, treat-ment group, and control group, with 10 rats in each group. GAD model was established by using the stimulation of uncertain empty drinking bottle. Then the treatment group and control group were intragas-trically given Danzhi Xiaoyao San ( Cortex Moutan and Fructus Gardeniae Free Wanderer Powder ) and Buspirone Hydrochloride once a day for 7 consecutive days respectively. The model group was given dis-tilled water once a day for a week. The expressions of bax and bcl-2 in rat hippocampus and mamillary
body were measured by applying western blot assay after resecting rat brains. Results The expression of bax in hippocampus and mamillary body of model group increased significantly compared with normal group ( P<0 . 05 or P<0 . 01 ) and the expression of bcl-2 decreased markedly ( P<0 . 01 ) . Compared with model group, the level of bax in hippocampus and mamillary body of treatment group and control group was reduced ( P<0 . 05 or P<0 . 01 ) and the level of bcl-2 increased significantly ( P<0 . 05 or P<0 . 01 ) . Conclusion The therapeutic method of liver-soothing heat-clearing and spleen-fortifying method represented by Danzhi Xiaoyao San could reduce the level of bax in hippocampus and mamillary body of GAD rat brain, elevate the level of bcl-2 and reduce bax/bcl-2, prevent and inhibit cell apoptosis in Papez’ s circuit and then ameliorate anxiety symptoms.
