The immortalized cell lines induced by hunan papillomavirus type 16 (HPVS 16) YY1 binding-site mu-tants have some characteristics of transformed cells.
10.3760/cma.j.issn.1003-9279.2000.03.001
- VernacularTitle:HPV 16 YY1位点突变株诱导的永生化细胞系具有部分转化细胞特征
- Author:
Hong LIU
1
;
Xiaoping DONG
;
Wei ZHOU
Author Information
1. Beijing Stoma-tological Hospital The C apital University of Medical Sciences
- Keywords:
Human papillomavirus;
YY1;
Telomerase;
Anchorage-independent growth
- From:
Chinese Journal of Experimental and Clinical Virology
2000;14(3):205-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological characteristics of the immortalized cell lines of hu ker-atinocytes from foreskin induced by humsn papillomavirus type 16 (HPV 16) YY1 binding- site mutants. Methods The cellular extracts of immortalized cell lines were prepared and the cellular endogenous p53 proteins were de-termined with Western blot. The cellular telomerase activities were analyzed with TRAP The immortalized cells wereincubated in a soft- agaarose medium containing 10% FCS and the anchorage-independent growth abilities of thetested cell lines were evaluated. Results Western blot showed that the endogenous p53 protein in all 4 testedclones were undetectable.The telomerases were clearly identified within the 4 tested cell lines,and showed an in-creasing activity along with the passages of the cells. All the clones were unable to grow in the soft- agarose medi-um during the earlier passges,but three of them showed anchorage - independent - growth at the passages of 30 -35.Conclusion The immortalized cell slines induced by the HPV 16 YY1 binding- site mutantshave activetelomerase activity and ability of anchorage- independent growth.
