Intervention of Feixian Formula extract on pulmonary microvascular endothelial cell migration and angiogenesis in vitro in rat model of pulmo-nary fibrosis

VernacularTitle:肺纤方提取物干预肺纤维化模型大鼠肺微血管内皮细胞新生血管形成的体外研究?
Author: Zhe LIU ¹ ; Xiao-Mei ZHANG ; Ting YIN ; Hui-Hui QIN ; Li-Yuan ZHANG ; Liang CHEN ; Yan-Fang HUANG
Author Information

1. 北京中医药大学北京100029
Keywords: Feixian Formula extract; pulmonary microvascular endothelial cells (PMVECs); cell migration; angiogenesis; rats
From: Journal of Beijing University of Traditional Chinese Medicine 2015;(4):233-235,252
CountryChina
Language:Chinese
Abstract: Objective To probe into the mechanism of Feixian Formula ( FXF ) , a Chinese herbal compound, in prevention pulmonary microvascular endothelial cells ( PMVECs) migration and angiogene-sis in vitro. Methods PMVECs isolated from rat model of pulmonary fibrosis ( PF) were cultured and divided into blank group, losartan group, prednisone group, low-, mid-, and high-dose of FXF groups. Migrating cells and new vessels in each group were counted. Results High-dose and mid-dose of FXF and losartan inhibited PMVECs migration significantly ( P <0. 05 ) . High-dose, mid-dose, low-dose of FXF and losartan inhibited formation of new vessels, even high-dose of FXF completely inhibited blood vessel formation. Conclusion The potential mechanism of anti-pulmonary fibrosis of FXF in vitro is related to inhabitation of PMVECs migration and new vessels formation.