Quantity determination of puerarin,daidzin and paeoniflorin in effective fraction of Qisho Formula by HPLC-PDA
- VernacularTitle:HPLC-PDA法测定芪芍方有效部位中葛根素、大豆苷、芍药苷的含量
- Author:
Li-Ping DAI
1
;
Ren-Bing SHI
Author Information
1. 河南中医学院
- Keywords:
Qisho Formula;
puerarin;
daidzin;
paeoniflorin;
HPLC
- From:
Journal of Beijing University of Traditional Chinese Medicine
2010;33(3):200-202,206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of HPLC-PDA for quantitative determination of puerarin,daidzin and paeoniflorin respectively in the effective fraction of Qisho Formula at the maximum absorption wavelength.Methods The procedure of HPLC-PDA was performed on the chromatographic column of ODSHYPERSIL(4.6 mm×250 mm,51μm),and the mobile phase in the gradient elution was acetonitrile-phosphoric acid solution(0.2%).The temperature was 35℃,the flow rate was 0.8 mL/min,and the detection wavelength was 250 nm and 230 nm respectively.Results Puerarin,daidzin and paeoniflorin showed good linear relationship,respectively,at range from 0.096 4 μg to 1.542 4 μg,0.094 4 μg to 1.510 4 μg and 0.099 6μg to 1.593 6μg.The average recovery was 97.44%,98.40% and 100.26% respectively(RSD=1.31%,2.70% and 1.35% respectively).Conclusion The method is easy and accurate with higher repeatability,which can be used in the quality control of the effective fraction of Qisho Formula.
