Etiological characteristics of Brucella melitensis in Henan Province, 2013-2022
10.3760/cma.j.cn112338-20240507-00244
- VernacularTitle:河南省2013-2022年羊种布鲁氏菌病原学特征分析
- Author:
Jiayong ZHAO
1
;
Weirong SONG
;
Baifan ZHANG
;
Yingxin HU
;
Yafei LI
;
Jingjing PAN
;
Haifeng WANG
;
Wanshen GUO
;
Xueyong HUANG
;
Ying YE
Author Information
1. 河南省疾病预防控制中心传染病预防控制所，郑州 450016
- Keywords:
Brucella melitensis;
Whole genome sequence;
Virulence;
Drug resistance;
Single nucleotide polymorphism;
Phylogenetic tree
- From:
Chinese Journal of Epidemiology
2024;45(11):1559-1565
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genus, drug resistance/virulence and phylogenetic characteristics of Brucella strains isolated from brucellosis surveillance sentinels in Henan Province from 2013 to 2022, and provide baseline data for the surveillance, early warning and outbreak tracing of brucellosis. Methods:Blood samples were collected from patients with Brucella infection for strain isolation, culture and species identification, drug susceptibility test, whole genome sequencing, splicing and assembly, functional/virulence/resistance gene prediction analysis and phylogenetic tree drawing based on single nucleotide polymorphism (SNP). Results:In 36 brucellosis patients, the majority were men (86.11%, 31/36), young adults aged 18-50 (88.89%, 32/36) and farmers/herdsmen (72.22%, 26/36). A total of 36 strains of Brucella melitensis were isolated, and average 1 305 functional proteins of 21 categories were predicted by strain genome; all the strains carried four main virulence factors (pmm, VirB group, BtpA/BtpB, BvrS/BvrR). The drug sensitivity rate was 100.00% to six types of antibiotics including levofloxacin, rifampicin, doxycycline, streptomycin, tetracycline and gentamicin, they showed different resistances to three antibiotics including compound trimethoprim-sulfamethoxazole, ciprofloxacin and ampicillin. The strains carried four types of resistance genes and two clusters of resistance genes, with four combinations of genotypes, the resistance mechanisms included antibiotic degradation/modification enzymes, resistant nodular cell differentiation (RND) efflux pumps, 16S/23S ribosomal rRNA binding site mutations, etc. The number of SNP differed in the genomes of 36 Brucellamelitensis strains ranged from 0 to 454 and phylogenetic tree was divided into three major branches, with relative branch distances between 0.000 0 and 0.498 6 for each strain. Conclusions:Human Brucellamelitensis strains isolated from surveillance sentinels in Henan from 2013 to 2022 carried multiple virulence and antibiotic resistance genes and had different drug resistance phenotypes. Single nucleotide polymorphism analysis and phylogenetic tree analysis showed significant differences in phylogenetic relationships among different strains.
