Effect of daidzein on proliferation of endometrial carcinoma

VernacularTitle:大豆甙元对子宫内膜癌细胞增殖的影响及其机制研究
Author: Min GAO ¹ ; Yuan-Kui CAO ; Xiao-Ou XUE
Author Information

1. 北京中医药大学东直门医院
Keywords: daidzein; endometrial carcinoma; proliferation
From: Journal of Beijing University of Traditional Chinese Medicine 2010;33(3):162-165
CountryChina
Language:Chinese
Abstract: Objective To study the effect of daidzein on the proliferation of endometrial carcinoma and the mechanism,and to provide the theoretical basis for the application of phytoestrogen.Methods Endometrial carcinoma cell line-Ishikawa was cultured in vitro.The influences of daidzein in different doses on the proliferation of Ishikawa cells were detected by using MTT method,and the changes of estrogen-related receptor-or-mRNA(ERRα-mRNA)and protein level were detected by applying RT-PCR and Western blot method.Results Daidzein inhibited significandy the proliferation of Ishikawa cells in vitro,and the effect was strengthened along with the increase of the dose and the prolong of action time,showing a dose-dependence and time-dependence.Daidzein in low dose(＜40 mmol/L)had no effects on ERRΑ mRNA and protein level,but in high dose(≥40 mmol/L)it regulated upward the expression of ERRα.Conclusion Daidzein can inhibit the proliferation of endometrial carcinoma through regulating the expression of ERRα.