Quantitative determination of caffeic acid in Dongkuiguo (Fructus Malvae, Mongolian medicated herb) by HPLC
- VernacularTitle:高效液相色谱法测定蒙药冬葵果中咖啡酸的含量
- Author:
Yu DONG
1
;
Qiang MA
;
Sheng-Sang NA
;
Xia LI
;
Shu-Ming LI
Author Information
1. 内蒙古医学院
- Keywords:
Dongkuiguo (Fructus Malvae,Mongolian medicated herb);
caffeic acid;
HPLC;
quantitative determination
- From:
Journal of Beijing University of Traditional Chinese Medicine
2010;33(2):117-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the quantitative determination of caffeic acid in Dongkuiguo (Fructus Malvae, Mongolian medicated herb) by RP-HPLC.Methods The procedure of RP-HPLC was performed on the chromatographic column of kromasil ODS C_(18) (250 mm×4.6 mm, 5 μm), and the mobile phase in the isocratic elution was methanol-1% acetic acid (18 to 82). The flow rate was 1.0 mL/min, the detection wavelength was 330 nm, and the temperature was at 30 ℃.Results Coffeic acid showed a good linear relationship at a range from 0.0275 2 μg to 0.344 0 μg, and the average recovery was 98.85% (RSD=2.29%, n=6).Conclusion The result of the quantitative determination shows that the method is simple and accurate, which is suitable for the quantitative determination of caffeic acid in Dongkuiguo.
